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Part:BBa_M36118:Design

Designed by: Samson Phan, Noriko Ishisoko   Group: Stanford BIOE44 - S11   (2012-05-29)


GULO enzyme with start and stop


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 558
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 558
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1307
    Illegal BamHI site found at 283
    Illegal BamHI site found at 1286
    Illegal XhoI site found at 993
    Illegal XhoI site found at 1317
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 558
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 558
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

sequence was slightly changed (silent mutation) from published work in order to allow for the use of restriction enzymes.


Source

mouse

References